SEMESTER III

 

CORE COURSE 3

CELL BIOLOGY, GENETICS AND MICROBIOLOGY- BOTTG-V THEORETICAL

(Credits 4, Lectures 60)

Cell Biology and Genetics

1. Ultrastructure   of   nuclear    envelope,    nucleolus    and   their    functions,   1.2    Molecular organisation of metaphase chromosome (Nucleosome concept).
2. Chromosomal aberrations- 2.1 deletion,duplication, inversion & translocation, 2.2 Aneuploidy &Polyploidy-types, importance and role in evolution.
3. Central Dogma,3.1 Transcription and Translation.
4. Genetic Code- properties.
5. Linkage groupand Genetic map (three-point test cross).
6. Mutation – 6.1 Point mutation(tautomerisation; transition, transversion and frame shift),6.2 Mutagen-physical and chemical.
7. Brief conceptof Split gene, Transposons.
8. Microbes
9. Viruses- Discovery, general structure, replication (general account), DNA virus (T-phage); Lytic and lysogenic cycle, RNA virus (TMV); Economic importance; 2.2 Bacteria- discovery, general characteristics and cell structure; reproduction- vegetative, asexual and recombination (conjugation, transformation and transduction); Economic importance

 

PRACTICAL- CELL BIOLOGY, GENETICSAND MICROBIOLOGY- BOTPG-VI

(Credits 2)

 

1. Cell Biology: Staining (Aceto-orcein) and squash preparation of onion root tip: study of mitoticstages. Determination of mitotic index (from onion root tip).
2. Microbiology: Workout gram staining (curd/any natural source)
3. Identification with reasons: Cytological slides of different mitotic and meiotic stages. Different forms ofbacteria (Coccus, Bacillus, Spiral)
4. Laboratory Records: Laboratory note books (regularly signed) and slides (prepared in class) are to be submitted at the time of Practical Examination. Regular attendance in the class must be credited